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goat polyclonal antibody against mouse rat myd88  (R&D Systems)


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    Structured Review

    R&D Systems goat polyclonal antibody against mouse rat myd88
    RFA induces <t>TIRAP/MyD88</t> but not TRAM/TRIF association. (A–D) . Mice was subjected to RFA or Sham treatment or ID injection of 5 µg LPS. Skin was collected 6 h later and subjected to cryo-sectioning and PLA analysis of close association of MyD88 and TIRAP (A, B) as well as TRIF and TRAM (C, D) . Representative PLA images were shown in (A, C) and quantitative results were shown in (B, D) . Arrows point to PLA signals. E. Skin sections in RFA groups in the above studies were also stained with fluorescence-conjugated anti-MHC II antibodies. Z-stack pictures were captured and used to create 3D images. Representative 3D pictures showing the overlapping of RFA-induced TIRAP/MyD88 PLA signals with MHC II. Arrows point to overlapping signals (yellow). (F) Pie chart of PLA signals overlapped with MHC II (blue). Scale: 100 µm in (A, C, E) . One-way ANOVA with Newman-Keuls multiple comparison test was used to compare differences between groups in (B, D) . n=14–20 in (B) and n=6–8 in (D) . Over 50 PLA signals were explored by investigators in (F) . *, p<0.05; **, p<0.01; ***, p<0.001. Data are representative of three independent experiments with similar results.
    Goat Polyclonal Antibody Against Mouse Rat Myd88, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+mouse+rat+myd88/pmc12528137-53-0-10?v=R%26D+Systems
    Average 93 stars, based on 41 article reviews
    goat polyclonal antibody against mouse rat myd88 - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Dual roles of in situ generated HSP70 in antigen delivery and immunoregulation"

    Article Title: Dual roles of in situ generated HSP70 in antigen delivery and immunoregulation

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1638948

    RFA induces TIRAP/MyD88 but not TRAM/TRIF association. (A–D) . Mice was subjected to RFA or Sham treatment or ID injection of 5 µg LPS. Skin was collected 6 h later and subjected to cryo-sectioning and PLA analysis of close association of MyD88 and TIRAP (A, B) as well as TRIF and TRAM (C, D) . Representative PLA images were shown in (A, C) and quantitative results were shown in (B, D) . Arrows point to PLA signals. E. Skin sections in RFA groups in the above studies were also stained with fluorescence-conjugated anti-MHC II antibodies. Z-stack pictures were captured and used to create 3D images. Representative 3D pictures showing the overlapping of RFA-induced TIRAP/MyD88 PLA signals with MHC II. Arrows point to overlapping signals (yellow). (F) Pie chart of PLA signals overlapped with MHC II (blue). Scale: 100 µm in (A, C, E) . One-way ANOVA with Newman-Keuls multiple comparison test was used to compare differences between groups in (B, D) . n=14–20 in (B) and n=6–8 in (D) . Over 50 PLA signals were explored by investigators in (F) . *, p<0.05; **, p<0.01; ***, p<0.001. Data are representative of three independent experiments with similar results.
    Figure Legend Snippet: RFA induces TIRAP/MyD88 but not TRAM/TRIF association. (A–D) . Mice was subjected to RFA or Sham treatment or ID injection of 5 µg LPS. Skin was collected 6 h later and subjected to cryo-sectioning and PLA analysis of close association of MyD88 and TIRAP (A, B) as well as TRIF and TRAM (C, D) . Representative PLA images were shown in (A, C) and quantitative results were shown in (B, D) . Arrows point to PLA signals. E. Skin sections in RFA groups in the above studies were also stained with fluorescence-conjugated anti-MHC II antibodies. Z-stack pictures were captured and used to create 3D images. Representative 3D pictures showing the overlapping of RFA-induced TIRAP/MyD88 PLA signals with MHC II. Arrows point to overlapping signals (yellow). (F) Pie chart of PLA signals overlapped with MHC II (blue). Scale: 100 µm in (A, C, E) . One-way ANOVA with Newman-Keuls multiple comparison test was used to compare differences between groups in (B, D) . n=14–20 in (B) and n=6–8 in (D) . Over 50 PLA signals were explored by investigators in (F) . *, p<0.05; **, p<0.01; ***, p<0.001. Data are representative of three independent experiments with similar results.

    Techniques Used: Injection, Staining, Fluorescence, Comparison

    HSP70 suppresses RFA-induced TLR4/IRAK/NFκB signaling. (A, B) WT and HSP70 KO mice were subjected to RFA or Sham treatment or ID injection of LPS or PBS. Skin was collected 6 h later in (A, B) IP and IB were conducted to evaluate TLR4/TIRAP binding (A) and IRAK4/IRAK1 binding in (B, C) . WT, HSP70 KO, MyD88 KO, TLR2 KO, and TLR4 KO mice were subjected to RFA or Sham treatment or ID injection of LPS. Skin was collected 2 h later. Cytosol and nuclear fractions were separated and nuclear fraction was analyzed by WB analysis to detect phosphorylated p65 using Lamin b1 as a loading control. (D) Skin IL-6 levels 6 h after RFA, Sham, or LPS treatment of lateral back skin of WT, HSP70 KO, TLR2 KO, TLR4 KO, and MyD88 KO mice. Two-way ANOVA with Fisher’s LSD test was used to compare differences between groups. n=4-6. *, p<0.05; **, p<0.01; ***, p<0.001. Original membrane pictures were shown in <xref ref-type= Supplementary Figure S9 . Data are representative of two independent experiments with similar results. " title="... binding in (B, C) . WT, HSP70 KO, MyD88 KO, TLR2 KO, and TLR4 KO mice were ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: HSP70 suppresses RFA-induced TLR4/IRAK/NFκB signaling. (A, B) WT and HSP70 KO mice were subjected to RFA or Sham treatment or ID injection of LPS or PBS. Skin was collected 6 h later in (A, B) IP and IB were conducted to evaluate TLR4/TIRAP binding (A) and IRAK4/IRAK1 binding in (B, C) . WT, HSP70 KO, MyD88 KO, TLR2 KO, and TLR4 KO mice were subjected to RFA or Sham treatment or ID injection of LPS. Skin was collected 2 h later. Cytosol and nuclear fractions were separated and nuclear fraction was analyzed by WB analysis to detect phosphorylated p65 using Lamin b1 as a loading control. (D) Skin IL-6 levels 6 h after RFA, Sham, or LPS treatment of lateral back skin of WT, HSP70 KO, TLR2 KO, TLR4 KO, and MyD88 KO mice. Two-way ANOVA with Fisher’s LSD test was used to compare differences between groups. n=4-6. *, p<0.05; **, p<0.01; ***, p<0.001. Original membrane pictures were shown in Supplementary Figure S9 . Data are representative of two independent experiments with similar results.

    Techniques Used: Injection, Binding Assay, Control, Membrane



    Similar Products

    goat polyclonal antibody against mouse rat myd88  (R&D Systems)
    93
    R&D Systems goat polyclonal antibody against mouse rat myd88
    RFA induces <t>TIRAP/MyD88</t> but not TRAM/TRIF association. (A–D) . Mice was subjected to RFA or Sham treatment or ID injection of 5 µg LPS. Skin was collected 6 h later and subjected to cryo-sectioning and PLA analysis of close association of MyD88 and TIRAP (A, B) as well as TRIF and TRAM (C, D) . Representative PLA images were shown in (A, C) and quantitative results were shown in (B, D) . Arrows point to PLA signals. E. Skin sections in RFA groups in the above studies were also stained with fluorescence-conjugated anti-MHC II antibodies. Z-stack pictures were captured and used to create 3D images. Representative 3D pictures showing the overlapping of RFA-induced TIRAP/MyD88 PLA signals with MHC II. Arrows point to overlapping signals (yellow). (F) Pie chart of PLA signals overlapped with MHC II (blue). Scale: 100 µm in (A, C, E) . One-way ANOVA with Newman-Keuls multiple comparison test was used to compare differences between groups in (B, D) . n=14–20 in (B) and n=6–8 in (D) . Over 50 PLA signals were explored by investigators in (F) . *, p<0.05; **, p<0.01; ***, p<0.001. Data are representative of three independent experiments with similar results.
    Goat Polyclonal Antibody Against Mouse Rat Myd88, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+mouse+rat+myd88/pmc12528137-53-0-10?v=R%26D+Systems
    Average 93 stars, based on 1 article reviews
    goat polyclonal antibody against mouse rat myd88 - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier
    goat polyclonal antibody against myd88  (R&D Systems)
    95
    R&D Systems goat polyclonal antibody against myd88
    RFA induces <t>TIRAP/MyD88</t> but not TRAM/TRIF association. (A–D) . Mice was subjected to RFA or Sham treatment or ID injection of 5 µg LPS. Skin was collected 6 h later and subjected to cryo-sectioning and PLA analysis of close association of MyD88 and TIRAP (A, B) as well as TRIF and TRAM (C, D) . Representative PLA images were shown in (A, C) and quantitative results were shown in (B, D) . Arrows point to PLA signals. E. Skin sections in RFA groups in the above studies were also stained with fluorescence-conjugated anti-MHC II antibodies. Z-stack pictures were captured and used to create 3D images. Representative 3D pictures showing the overlapping of RFA-induced TIRAP/MyD88 PLA signals with MHC II. Arrows point to overlapping signals (yellow). (F) Pie chart of PLA signals overlapped with MHC II (blue). Scale: 100 µm in (A, C, E) . One-way ANOVA with Newman-Keuls multiple comparison test was used to compare differences between groups in (B, D) . n=14–20 in (B) and n=6–8 in (D) . Over 50 PLA signals were explored by investigators in (F) . *, p<0.05; **, p<0.01; ***, p<0.001. Data are representative of three independent experiments with similar results.
    Goat Polyclonal Antibody Against Myd88, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+mouse+rat+myd88/pm22067617-77-65-70?v=R%26D+Systems
    Average 95 stars, based on 1 article reviews
    goat polyclonal antibody against myd88 - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    RFA induces TIRAP/MyD88 but not TRAM/TRIF association. (A–D) . Mice was subjected to RFA or Sham treatment or ID injection of 5 µg LPS. Skin was collected 6 h later and subjected to cryo-sectioning and PLA analysis of close association of MyD88 and TIRAP (A, B) as well as TRIF and TRAM (C, D) . Representative PLA images were shown in (A, C) and quantitative results were shown in (B, D) . Arrows point to PLA signals. E. Skin sections in RFA groups in the above studies were also stained with fluorescence-conjugated anti-MHC II antibodies. Z-stack pictures were captured and used to create 3D images. Representative 3D pictures showing the overlapping of RFA-induced TIRAP/MyD88 PLA signals with MHC II. Arrows point to overlapping signals (yellow). (F) Pie chart of PLA signals overlapped with MHC II (blue). Scale: 100 µm in (A, C, E) . One-way ANOVA with Newman-Keuls multiple comparison test was used to compare differences between groups in (B, D) . n=14–20 in (B) and n=6–8 in (D) . Over 50 PLA signals were explored by investigators in (F) . *, p<0.05; **, p<0.01; ***, p<0.001. Data are representative of three independent experiments with similar results.

    Journal: Frontiers in Immunology

    Article Title: Dual roles of in situ generated HSP70 in antigen delivery and immunoregulation

    doi: 10.3389/fimmu.2025.1638948

    Figure Lengend Snippet: RFA induces TIRAP/MyD88 but not TRAM/TRIF association. (A–D) . Mice was subjected to RFA or Sham treatment or ID injection of 5 µg LPS. Skin was collected 6 h later and subjected to cryo-sectioning and PLA analysis of close association of MyD88 and TIRAP (A, B) as well as TRIF and TRAM (C, D) . Representative PLA images were shown in (A, C) and quantitative results were shown in (B, D) . Arrows point to PLA signals. E. Skin sections in RFA groups in the above studies were also stained with fluorescence-conjugated anti-MHC II antibodies. Z-stack pictures were captured and used to create 3D images. Representative 3D pictures showing the overlapping of RFA-induced TIRAP/MyD88 PLA signals with MHC II. Arrows point to overlapping signals (yellow). (F) Pie chart of PLA signals overlapped with MHC II (blue). Scale: 100 µm in (A, C, E) . One-way ANOVA with Newman-Keuls multiple comparison test was used to compare differences between groups in (B, D) . n=14–20 in (B) and n=6–8 in (D) . Over 50 PLA signals were explored by investigators in (F) . *, p<0.05; **, p<0.01; ***, p<0.001. Data are representative of three independent experiments with similar results.

    Article Snippet: Goat polyclonal antibody against mouse/rat MyD88 (AF3109) was purchased from R & D Systems (Minneapolis, MN).

    Techniques: Injection, Staining, Fluorescence, Comparison

    HSP70 suppresses RFA-induced TLR4/IRAK/NFκB signaling. (A, B) WT and HSP70 KO mice were subjected to RFA or Sham treatment or ID injection of LPS or PBS. Skin was collected 6 h later in (A, B) IP and IB were conducted to evaluate TLR4/TIRAP binding (A) and IRAK4/IRAK1 binding in (B, C) . WT, HSP70 KO, MyD88 KO, TLR2 KO, and TLR4 KO mice were subjected to RFA or Sham treatment or ID injection of LPS. Skin was collected 2 h later. Cytosol and nuclear fractions were separated and nuclear fraction was analyzed by WB analysis to detect phosphorylated p65 using Lamin b1 as a loading control. (D) Skin IL-6 levels 6 h after RFA, Sham, or LPS treatment of lateral back skin of WT, HSP70 KO, TLR2 KO, TLR4 KO, and MyD88 KO mice. Two-way ANOVA with Fisher’s LSD test was used to compare differences between groups. n=4-6. *, p<0.05; **, p<0.01; ***, p<0.001. Original membrane pictures were shown in <xref ref-type= Supplementary Figure S9 . Data are representative of two independent experiments with similar results. " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Dual roles of in situ generated HSP70 in antigen delivery and immunoregulation

    doi: 10.3389/fimmu.2025.1638948

    Figure Lengend Snippet: HSP70 suppresses RFA-induced TLR4/IRAK/NFκB signaling. (A, B) WT and HSP70 KO mice were subjected to RFA or Sham treatment or ID injection of LPS or PBS. Skin was collected 6 h later in (A, B) IP and IB were conducted to evaluate TLR4/TIRAP binding (A) and IRAK4/IRAK1 binding in (B, C) . WT, HSP70 KO, MyD88 KO, TLR2 KO, and TLR4 KO mice were subjected to RFA or Sham treatment or ID injection of LPS. Skin was collected 2 h later. Cytosol and nuclear fractions were separated and nuclear fraction was analyzed by WB analysis to detect phosphorylated p65 using Lamin b1 as a loading control. (D) Skin IL-6 levels 6 h after RFA, Sham, or LPS treatment of lateral back skin of WT, HSP70 KO, TLR2 KO, TLR4 KO, and MyD88 KO mice. Two-way ANOVA with Fisher’s LSD test was used to compare differences between groups. n=4-6. *, p<0.05; **, p<0.01; ***, p<0.001. Original membrane pictures were shown in Supplementary Figure S9 . Data are representative of two independent experiments with similar results.

    Article Snippet: Goat polyclonal antibody against mouse/rat MyD88 (AF3109) was purchased from R & D Systems (Minneapolis, MN).

    Techniques: Injection, Binding Assay, Control, Membrane